ABSTRACT
Objective: To explore the biological properties of keratinocytes from differently-aged healthy human beings. Methods: Keratinocytes from fetus, teenager and middle-aged groups were separated and cultured. The population doubling time (PDT) and cell growth curve in different cells were compared, and the cell cycles were analyzed by flow cytometry. Results: Circled digit one In primary culture of keratinocytes, the adherence time in middle-aged group was longer than that in fetus and teenager groups. However, all cell morphology showed no obvious differences. In subculture of keratinocytes, with donator's age increasing, time of cell adherence prolonged, passage number decreased and differences in cell morphology were obvious. Circled digit two The average PDT of keratinocytes was shorter in fetus group than in teenager and middle-aged groups. But difference in cell growth curve between different passages was not observed. Circled digit three Keratinocytes showed G2/M period in fetus group but G0/G1 period in teenager and middle-aged groups mainly. Conclusion: As age increases, the biological properties of keratinocytes change obviously.
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of basic fibroblast growth factor (bFGF) and alpha-melanocyte stimulating hormone (alpha-MSH) on adhesion and migration of melanocytes in vitro.</p><p><b>METHODS</b>Human melanocytes were obtained from normal human foreskins. Culture dishes covered with fibronectin were used to perform melanocytes adhesion assay, and cell motility was assessed using the Transwell micropore filter method.</p><p><b>RESULT</b>bFGF and alpha-MSH increased melanocytes adhesion on culture dishes covered with fibronectin. bFGF stimulated melanocytes migration through micropore filter while alpha-MSH had no significant effects.</p><p><b>CONCLUSION</b>bFGF and alpha-MSH could promote the adhesion and migration of melanocytes, which suggests that two agents may play a role in the repigmentation of vitiligo.</p>
Subject(s)
Humans , Cell Adhesion , Cell Movement , Cells, Cultured , Fibroblast Growth Factor 2 , Pharmacology , Melanocytes , Cell Biology , alpha-MSH , PharmacologyABSTRACT
This article described the concept of the professional ethics and discussed the significance of the existence and construction of professional ethics on medical journal editors. The professional ethics on medical journal editors could be beneficial to correctly understand the ethical problems of medical journal editors and to promote the medical journal editors' role localization. It is very important to construct the Standardization of medical journal editors' behavior.